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77 questions
Biology/Paper 4/Use of Techniques, Apparatus and Materials
CAIEO-Level5090-o · Paper 4

Use of Techniques, Apparatus and Materials

77 questions· page 1 of 8

Q3(a)2014 Oct/Nov·P624MEasy
(a)

Complete Table 3.1.

Table 3.1

reagent usedcolour of reagent at the startfinal colour of reagentconclusion
Benedict's solutionbluetrace of reducing sugar present
biuretblueprotein present
iodine solutionblack
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Q32013 May/Jun·P627MMedium-Easy

The composition of inhaled air differs from that of exhaled air.

Complete Table 3.1 for water vapour in exhaled air and state the test for the presence of each of the three gases.

Table 3.1

inhaled airexhaled airtest
carbon dioxide0.04%4.0%
oxygen21%16.0%
water vapourvariable – depends on environment
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Q42012 Oct/Nov·P612 partsMedium-Easy
(a)

reducing sugar

______

(b)

protein.

______

Q4(a)2013 Oct/Nov·P614MMedium-Easy
(a)

Glucose is a reducing sugar.

Describe how you would test for glucose safely in your laboratory.

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Q22017 May/Jun·P622 partsEasy
(a)(i)

starch

(a)(ii)

reducing sugars

Q12017 Oct/Nov·P623 partsMedium-Easy
(a)

Describe how you would use Benedict's solution to test for reducing sugar in a piece of potato. Include details of a safety precaution in your answer.

(e)

You are given a 1.0% glucose solution. Describe in detail how you would use it to produce 5 cm35\text{ cm}^3 of a 0.5% glucose solution.

(f)

After the students had tested the glucose solutions in Table 1.1 with Benedict's solution, they noticed that a solid (precipitate) had collected at the bottom of the test-tubes.

They decided that finding the mass of the solid was another way of measuring the concentration of glucose in the solution.

Suggest how the students could separate this solid from the solution and obtain its mass.

Q12024 Oct/Nov·P423 partsEasy
(a)(i)

Suggest a suitable reagent for the test for starch and describe how it should be used.
Details of possible results are not required.

______

(b)(i)

Describe how you would keep the test-tubes at the required temperature for ten minutes.

______

(c)

Describe how you would test the apple for protein, and state what you would observe if protein was present and not present.

test ______

protein present ______
protein not present ______

Q2(a)2016 May/Jun·P625MMedium
(a)

Milk contains a reducing sugar. Explain how you could safely compare the reducing sugar contents of samples of cow's milk and goat's milk.

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Q3(a)2020 Oct/Nov·P623MMedium-Easy
(a)

Suggest a method that he could use to determine this volume of milk.

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Q2(b)2021 Oct/Nov·P615MMedium
(b)

Some of the outer cells in the photomicrograph contain small particles.

Describe in detail how you would prepare a section of the root for observation under the microscope to see whether these cells contain starch.

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