5090/32

Biology 5090/32October/November 2024

Cambridge O-Level · Practical Test · worked solutions for every part, with the mark scheme

3
questions
40
marks
90
minutes

Topics Observations and Measurements · Experimental Contexts · Analysis, Conclusions and Evaluation · Use of Techniques, Apparatus and Materials · Planning Experiments and Investigations · Microscopy and Biological Drawing

Q117MMediumUse of Techniques, Apparatus and MaterialsObservations and MeasurementsExperimental ContextsAnalysis, Conclusions and Evaluation

Carbohydrates are found in different plant organs in the form of starch and sugars (such as glucose and maltose).

You are going to test two different organs from two plants for the presence of starch and sugar.

You are provided with a piece of potato and a piece of apple.

(a)
6M
(i)

State which reagent you will use for the test for starch and describe how you will use it.

Details of possible results are not required.

______

2M
(ii)

Carry out a test for starch on a piece of the apple and a piece of the potato. Record your observations and what you can conclude from them in Table 1.1.

Table 1.1

plant organobservationconclusion
apple
potato
4M
(b)

You are going to use Benedict's solution to test for sugar in the potato and apple.

Follow these instructions:

  • Cut a 1cm×1cm×1cm1\,\text{cm} \times 1\,\text{cm} \times 1\,\text{cm} cube of potato.
  • Cut the cube into small pieces and place these in a large test-tube.
  • Label the test-tube.
  • Add 4cm34\,\text{cm}^3 of distilled water and use a stirring rod to gently crush the pieces and mix them with the water.
  • Clean your tile, cutting device and stirring rod.

Repeat this procedure with the apple using a clean test-tube.

  • Add 8cm38\,\text{cm}^3 of Benedict's solution to both test-tubes.

Then raise your hand and the supervisor will add hot water to your water-bath.

Take care as the water will be hot.

7M
(i)

Record the temperature of the water-bath at the start.

temperature at start = ______

1M
(ii)

Place both test-tubes in the water-bath and leave them for ten minutes.

Record your observations and your conclusions in Table 1.2 after the test-tubes have been in the water-bath for ten minutes.

Table 1.2

plant organobservationconclusion
apple
potato
2M
(iii)

Suggest why it was important to:

  • use a 1cm×1cm×1cm1\,\text{cm} \times 1\,\text{cm} \times 1\,\text{cm} cube for each plant organ

______

  • cut each cube up into small pieces

______

  • crush the small pieces and mix them with water.

______

3M
(iv)

Explain why the tile, cutting device and stirring rod were cleaned after using them on the potato.

______

1M
(c)

Describe how you would test the apple for protein, and state what you would observe if protein was present and not present.

test = ______
protein present = ______
protein not present = ______

4M
Q214MMedium-HardObservations and MeasurementsExperimental ContextsAnalysis, Conclusions and EvaluationPlanning Experiments and Investigations

Photosynthesis in green plants can be summarised by the equation:

carbon dioxide+waterlight andchlorophyllcarbohydrate+oxygen\text{carbon dioxide} + \text{water} \xrightarrow{\substack{\text{light and} \\ \text{chlorophyll}}} \text{carbohydrate} + \text{oxygen}

A student used the apparatus shown in Fig. 2.1 to investigate the rate of photosynthesis in an aquatic plant.

When the student switched the lamp on, bubbles of oxygen formed and entered the syringe. The student recorded the volume of oxygen produced in ten minutes. The syringe was then refilled with sodium hydrogencarbonate solution and the oxygen collected for another ten minutes.

The student then decided to investigate the effect of different colours of light on the rate of photosynthesis. Leaving the same plant in the glass jar, a red transparent filter was wrapped around the glass jar so that the plant received only red light. The volume of oxygen produced in ten minutes was measured. This measurement was then repeated.

The red transparent filter was replaced by a green transparent filter and then by a blue transparent filter. For each filter, the volume of oxygen produced in ten minutes was also measured twice.

The measurements are shown in Table 2.1

Table 2.1

filtervolume of gas collected in ten minutes / cm3\text{cm}^3
measurement 1measurement 2mean
no filter1.11.31.2
red0.70.90.8
green0.30.30.3
blue0.6
(a)

The syringe at the end of ten minutes for measurement 2 with the blue filter is shown in Fig. 2.2.

3M
(i)

Record the volume of oxygen produced in measurement 2 with the blue filter in Table 2.1.

1M
(ii)

Calculate the mean volume of oxygen produced with the blue filter and record it in Table 2.1.

1M
(iii)

Using the mean value, calculate the rate of photosynthesis per minute when no filter was used.

______

1M
(b)
5M
(i)

Construct a bar chart on the grid to show the mean volume of gas collected with no filter and with the different coloured filters.

4M
(ii)

Use the data given and the bar chart to state one conclusion that can be made from the results of this investigation.

______

1M
(c)

Plan an investigation that you could do to determine the effect of light intensity on the rate of photosynthesis, using the apparatus shown in Fig. 2.1.

6M
Q39MMediumExperimental ContextsObservations and MeasurementsMicroscopy and Biological DrawingAnalysis, Conclusions and EvaluationUse of Techniques, Apparatus and Materials

Fig. 3.1 shows photographs of a male and a female of an insect species. Both the male and female insects are green in colour.

(a)
8M
(i)

State one visible difference between the male and female in Fig. 3.1.

______

1M
(ii)

In the space below, make a large drawing of the male insect as it appears in Fig. 3.1.

4M
(iii)

C and D indicate the length of the female insect. D indicates the end of the abdomen.

Draw a line to join C and D on the photograph.

Measure the length of the line and record it.

______

Calculate the actual length of the insect and record it to the nearest whole number.

______ mm\text{mm}

3M
(b)

Use the key below to identify and record the name of the insect.

1 wings longer than abdomen ............. Oedemera femoralis
wings shorter than abdomen ............ go to 2

2 colour brown ..................................... Oedemera barbara
colour green or grey ......................... go to 3

3 length 5–6.5 mm .............................. Oedemera lurida
length 7–11 mm ................................ Oedemera nobilis

name of insect = ______

1M