5090/31

Biology 5090/31May/June 2014

Cambridge O-Level · Practical Test · worked solutions for every part, with the mark scheme

2
questions
40
marks
75
minutes

Topics Experimental Contexts · Observations and Measurements · Analysis, Conclusions and Evaluation · Planning Experiments and Investigations · Microscopy and Biological Drawing · Use of Techniques, Apparatus and Materials

Q113MExperimental ContextsObservations and MeasurementsAnalysis, Conclusions and EvaluationPlanning Experiments and InvestigationsFree sample

You are going to investigate the effect of sugar solution on onions.

You are provided with a transverse slice, 2 mm2\text{ mm} thick, that was cut from a whole onion.

  • Separate the outer ring from the others as shown in Fig. 1.1A.
  • Cut this ring into two equal halves as shown in Fig. 1.1B.

  • Place one piece in the dish containing distilled water and place the other piece in the dish containing sugar solution.
(a)
(i)

Draw the shape of these two pieces, at the start, in Table 1.1.
Show the position of the outer layer of onion on the drawing of each piece.

Table 1.1

shape of the piece: in distilled watershape of the piece: in sugar solution
at start
after 30 minutes
2M
(ii)

Leave the dishes for at least 30 minutes and proceed with Question 2.

After 30 minutes or more observe the two pieces of onion.
Draw the shape of these two pieces in Table 1.1 in the lower spaces.

2M
(iii)

Describe the change in the shape in the two pieces of onion after 30 minutes compared to the pieces at the start.

______

2M
(iv)

Explain what has happened to cause the changes in the pieces of onion.

______

5M
(b)

State one factor that was kept the same in this investigation and explain why it was kept the same.

______

2M

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1 more questions
  • Q2Microscopy and Biological Drawing · Experimental Contexts · Observations and Measurements · Use of Techniques, Apparatus and Materials · Analysis, Conclusions and Evaluation · Planning Experiments and Investigations27M
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